Generator

Part:BBa_K511907:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Inducible Mnt-VP16 Transactivator Generator (TRE-Tight-Mnt-VP16) MammoBlock Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 445
    Illegal XbaI site found at 30
    Illegal PstI site found at 458
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 445
    Illegal PstI site found at 458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 445
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 445
    Illegal XbaI site found at 30
    Illegal PstI site found at 458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 445
    Illegal XbaI site found at 30
    Illegal PstI site found at 458
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.



Source

Consult constituent components.

References