Plasmid_Backbone

Part:BBa_K5119036:Design

Designed by: Francesca Abraham   Group: iGEM24_Austin-utexas   (2024-09-27)


pIB184-GFP Shuttle Plasmid Backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 363
    Illegal XbaI site found at 367
    Illegal XbaI site found at 5273
    Illegal XbaI site found at 6321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 363
    Illegal NheI site found at 981
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 363
    Illegal BglII site found at 378
    Illegal BamHI site found at 344
    Illegal XhoI site found at 374
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 363
    Illegal XbaI site found at 367
    Illegal XbaI site found at 5273
    Illegal XbaI site found at 6321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 363
    Illegal XbaI site found at 367
    Illegal XbaI site found at 5273
    Illegal XbaI site found at 6321
    Illegal NgoMIV site found at 101
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

This plasmid had to be domesticated to remove illegal restriction sites outside the dropout region. An sfGFP gene dropout was added for use in GGA. An RP4 oriT sequence was added to enable conjugative transfer of the plasmid.

Source

The genomic sequence of this plasmid was modified from an existing plasmid, pIB184 (EM), as a gift from Indranil Biswas (Addgene plasmid # 90194 ; http://n2t.net/addgene:90194 ; RRID:Addgene_90194).

References