Generator
Part:BBa_K511901:Design
Designed by: Grant Robinson Group: iGEM11_MIT (2011-09-28)
LacI Repressor Generator (Hef1a-LacI) MammoBlock Device
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal BglII site found at 615
Illegal XhoI site found at 1014 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
Illegal NgoMIV site found at 749
Illegal AgeI site found at 127 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.
Source
Consult constituent components.