Generator
Part:BBa_K511823:Design
Designed by: Grant Robinson Group: iGEM11_MIT (2011-09-28)
Inducible Red Fluorescent Protein Generator (UAS-Gal4-mKate) MammoBlock Device
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 126
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 126
Illegal NheI site found at 154 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 126
Illegal XhoI site found at 83 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 126
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 126
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 876
Illegal SapI.rc site found at 258
Design Notes
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.
Source
Consult constituent components.