Signalling

Part:BBa_K511814:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Inducible Red Fluorescent Ligand Generator (TRE-Tight-Delta-mCherry) MammoBlock Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 437
    Illegal PstI site found at 514
    Illegal PstI site found at 1357
    Illegal PstI site found at 1780
    Illegal PstI site found at 2457
    Illegal PstI site found at 2843
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal PstI site found at 437
    Illegal PstI site found at 514
    Illegal PstI site found at 1357
    Illegal PstI site found at 1780
    Illegal PstI site found at 2457
    Illegal PstI site found at 2843
    Illegal NotI site found at 846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 437
    Illegal PstI site found at 514
    Illegal PstI site found at 1357
    Illegal PstI site found at 1780
    Illegal PstI site found at 2457
    Illegal PstI site found at 2843
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 437
    Illegal PstI site found at 514
    Illegal PstI site found at 1357
    Illegal PstI site found at 1780
    Illegal PstI site found at 2457
    Illegal PstI site found at 2843
    Illegal AgeI site found at 873
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 385
    Illegal BsaI site found at 825
    Illegal BsaI.rc site found at 2090
    Illegal BsaI.rc site found at 2262
    Illegal SapI site found at 1578
    Illegal SapI.rc site found at 2350


Design Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.



Source

Consult constituent components.

References