Generator
Part:BBa_K511807
Designed by: Grant Robinson Group: iGEM11_MIT (2011-09-28)
Repressible Red Fluorescent Protein Generator (Hef1a-LacO-mKate) MammoBlock Device
This MammoBlock composite device produces the monomeric red fluorescent protein mKate at a low, constitutive level driven by the Hef1a-LacO promoter and repressible by LacI variants.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal BglII site found at 615
Illegal XhoI site found at 1014 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
Illegal NgoMIV site found at 749
Illegal AgeI site found at 127 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1913
Illegal SapI.rc site found at 1295
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Categories
Parameters
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