Composite

Part:BBa_K5117048:Design

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-09-28)


PcotYZ-BsRBS-AtCelO-L2-CotY-B0014


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2170
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,

removal of signal peptide (except start codon ATG) and stop codon of AtCelO,

addition of a flexible linker encoding the amino acids (GGGGS)4,

addition of a 10 bp spacer downstream the CotY coding sequence

Source

Bacillus subtilis and Acetivibrio thermocellus