Composite

Part:BBa_K5117040:Design

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-09-28)


PcotYZ-BsRBS-BhBglA-L3-CotY-B0014


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 764
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1597
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,

exchange of start codon (GTG > ATG) and removal of stop codon of BhBglA,

addition of a rigid linker encoding the amino acids GGGEAAAKGGG,

addition of a 10 bp spacer downstream the CotY coding sequence

Source

Bacillus subtilis and Bacillus halodurans