Coding

Part:BBa_K5117022:Design

Designed by: Jenny Sauermann   Group: iGEM24_TU-Dresden   (2024-09-28)


CotY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 23
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

addition of a 10 bp long spacer (TCATAAGCTG) downstream of the coding sequence

Source

Bacillus subtilis