Coding

Part:BBa_K511701:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)


B-Arrestin-2-TEV-Protease MammoBlock


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 307
    Illegal PstI site found at 394
    Illegal PstI site found at 1450
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 307
    Illegal PstI site found at 394
    Illegal PstI site found at 1450
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 604
    Illegal XhoI site found at 998
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 307
    Illegal PstI site found at 394
    Illegal PstI site found at 1450
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 307
    Illegal PstI site found at 394
    Illegal PstI site found at 1450
    Illegal AgeI site found at 590
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 865
    Illegal BsaI site found at 1830


Design Notes

Not applicable.


Source

The N1a domain was isolated from the tobacco etch mosaic virus while the B-Arrestin-2 protein was isolated from a human cDNA library.


References