Composite

Part:BBa_K5117017:Design

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-08-31)


BsRBS-BhBglA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 579
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,

exchange of start codon (GTG > ATG) for usage in Bacillus subtilis as host organism

Source

Bacillus halodurans