Coding

Part:BBa_K511600:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)

N-Cadherin-2A-EYFP Cell Adhesion MammoBlock


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2723
    Illegal PstI site found at 287
    Illegal PstI site found at 1388
    Illegal PstI site found at 2999
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2723
    Illegal PstI site found at 287
    Illegal PstI site found at 1388
    Illegal PstI site found at 2999
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2723
    Illegal PstI site found at 287
    Illegal PstI site found at 1388
    Illegal PstI site found at 2999
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2723
    Illegal PstI site found at 287
    Illegal PstI site found at 1388
    Illegal PstI site found at 2999
    Illegal NgoMIV site found at 1239
    Illegal NgoMIV site found at 2765
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1274
    Illegal BsaI.rc site found at 1831


Design Notes

Not applicable.


Source

The N-Cadherin sequence of this part was isolated from a murine source; the viral 2A self-cleaving peptide was isolated from a virus with homology to the foot-and-mouth disease virus; the EYFP fluorescent reporter is an engineered version of the original green fluorescent protein from Aequorea victoria. Sincere thanks go to the Weiss lab for contributing this entry vector to the MIT iGEM 2011 team.

References