Part:BBa_K5115075

nikD

Introduction

The nikD is a subunit of the nik operon BBa_K2652006.

Usage and Biology

NikD is a membrane-associated protein with ATPase activity in the NikABCDE complex. It provides the energy required for nickel transport by hydrolyzing ATP. In our design, nikD is constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115080(ribozyme+RBS+nikD+stem-loop), eventually making up parts BBa_K5115082(ribozyme connected nik operon), which is used for further experiments to facilitate nickel uptake in E. coli.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

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• 1000
  COMPATIBLE WITH RFC[1000]