Composite

Part:BBa_K5115064:Design

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-09-19)


hox and hyp, with EP targeted hoxU


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 654
    Illegal BglII site found at 1472
    Illegal BglII site found at 1765
    Illegal BglII site found at 10143
    Illegal BglII site found at 10221
    Illegal BamHI site found at 2059
    Illegal XhoI site found at 99
    Illegal XhoI site found at 2266
    Illegal XhoI site found at 4505
    Illegal XhoI site found at 6334
    Illegal XhoI site found at 7496
    Illegal XhoI site found at 9335
    Illegal XhoI site found at 10151
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 1071
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1593
    Illegal NgoMIV site found at 2359
    Illegal NgoMIV site found at 3039
    Illegal NgoMIV site found at 5646
    Illegal AgeI site found at 6821
    Illegal AgeI site found at 10104
    Illegal AgeI site found at 11103
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4159
    Illegal BsaI site found at 9925
    Illegal BsaI site found at 10087
    Illegal BsaI.rc site found at 702
    Illegal BsaI.rc site found at 1206
    Illegal SapI.rc site found at 10036


Design Notes

none


Source

synthetic

References