Part:BBa_K5115058

hoxF fused with EP

Introduction

Combine hoxF directly with EP. The hoxF is a subunit of the hydrogenase, check it in BBa_K5115020(hox and hyp operon). EP can direct external proteins into carboxysomes, check it in BBa_K51150002(EP).

Usage and Biology

Binding hoxF with EP can direct the hydrogenase into the carboxysome, creating a safe and stable environment for the enzyme to work.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 577
  Illegal BglII site found at 1395
  Illegal BglII site found at 1688
  Illegal XhoI site found at 22
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 456
  Illegal NgoMIV site found at 994
  Illegal NgoMIV site found at 1204
  Illegal NgoMIV site found at 1516
  Illegal AgeI site found at 2471
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 625
  Illegal BsaI.rc site found at 1129