Coding
Part:BBa_K5115004
Designed by: Liyue Chen Group: iGEM24_Fudan (2024-09-18)
hoxY
Introduction
The hoxY is a subunit of the hox and hyp operon BBa_K5115020.
Usage and Biology
It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115013(ribozyme+RBS+hoxY+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 57
Illegal XhoI site found at 264 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 357
- 1000COMPATIBLE WITH RFC[1000]
References
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Categories
Parameters
None |