Part:BBa_K5115003

hoxU, without stop codon

Introduction

The hoxU is a subunit of the hox and hyp operon BBa_K5115020.

Usage and Biology

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115012(ribozyme+RBS+hoxU+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 410
  Illegal BglII site found at 488
  Illegal XhoI site found at 418
  Illegal XhoI site found at 610
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 371
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 192
  Illegal BsaI site found at 354
  Illegal SapI.rc site found at 303