Part:BBa_K511303

Delta-mCherry-4xFF4 Juxtacrine Signaling Ligand MammoBlock

This part encodes a fusion between the Delta-3 ligand and the monomeric mCherry red fluorescent protein. Additionally, the protein is succeeded by four copies of the FF4 intronic miRNA binding sequence, allowing for miRNA-mediated repression of this part's translation.

Figures 1 and 2 below show fluorescent output from the Delta-mCherry protein, while Figures 3 and 4 below show transactivation of the Notch-Gal4-ESN receptor by the Delta-mCherry protein.

For more information, consult the MIT iGEM 2011 wiki here.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 85
  Illegal PstI site found at 162
  Illegal PstI site found at 1005
  Illegal PstI site found at 1428
  Illegal PstI site found at 2105
  Illegal PstI site found at 2491
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 85
  Illegal PstI site found at 162
  Illegal PstI site found at 1005
  Illegal PstI site found at 1428
  Illegal PstI site found at 2105
  Illegal PstI site found at 2491
  Illegal NotI site found at 494
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 85
  Illegal PstI site found at 162
  Illegal PstI site found at 1005
  Illegal PstI site found at 1428
  Illegal PstI site found at 2105
  Illegal PstI site found at 2491
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 85
  Illegal PstI site found at 162
  Illegal PstI site found at 1005
  Illegal PstI site found at 1428
  Illegal PstI site found at 2105
  Illegal PstI site found at 2491
  Illegal AgeI site found at 521
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 33
  Illegal BsaI site found at 473
  Illegal BsaI.rc site found at 1738
  Illegal BsaI.rc site found at 1910
  Illegal SapI site found at 1226
  Illegal SapI.rc site found at 1998