Coding

Part:BBa_K5111007

Designed by: Thiago Espurio Moraes   Group: iGEM24_City-of-London-UK   (2024-09-28)

Tat-dependent export supporter

Nitrous oxide reductase (N2OR) is a periplasmic protein that is assembled in the cytoplasm and exported to the periplasm via a TAT system. The tatE protein supports the Tat-dependent export of N2OR to the periplasm (Zhang et al., 2019). This is essential in Clade I organisms as the system for N2OR must be periplasmic to properly function (Bennett et al., 2019).

Sources

Zhang, L., Wüst, A., Prasser, B., Müller, C. and Einsle, O. (2019a). Functional assembly of nitrous oxide reductase provides insights into copper site maturation. Proceedings of the National Academy of Sciences, 116(26), pp.12822–12827. doi:https://doi.org/10.1073/pnas.1903819116.

Bennett, S.P., Soriano-Laguna, M.J., Bradley, J.M., Svistunenko, D.A., Richardson, D.J., Gates, A.J. and Le, N.E. (2019). NosL is a dedicated copper chaperone for assembly of the CuZcenter of nitrous oxide reductase. Chemical Science, 10(19), pp.4985–4993. doi:https://doi.org/10.1039/c9sc01053j.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None