Part:BBa_K5111006

FAD:protein FMN transferase

ApbE is the FAD+ binding flavinyl transferase protein that donates flavin to the NosR protein for maturation, which then activates the protein N2OR (Zhang et al., 2017). Most N2OR-containing bacteria code for NosX, a homologue of ApbE (Bennett et al., 2020). This is not the case for the bacteria we chose to take the nos gene cluster from, P.stutzeri (Clade I) ZoBell ATCC 14405.

Sources

Bennett, S.P. et al. (2020) 'nosX is essential for whole-cell N2O reduction in Paracoccus denitrificans but not for assembly of copper centres of nitrous oxide reductase,' Microbiology, 166(10), pp. 909–917. https://doi.org/10.1099/mic.0.000955

Lin Zhang, Christian Trncik, Susana L.A. Andrade, Oliver Einsle, (2017). The flavinyl transferase ApbE of Pseudomonas stutzeri matures the NosR protein required for nitrous oxide reduction. Biochimica et Biophysica Acta (BBA) – Bioenergetics, Volume 1858, Issue 2, Pages 95-102, ISSN 0005-2728. doi: https://doi.org/10.1016/j.bbabio.2016.11.008

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 684
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 153
  Illegal NgoMIV site found at 205
  
• 1000
  COMPATIBLE WITH RFC[1000]