Coding
Part:BBa_K5111005:Design
Designed by: Angus Lusty Group: iGEM24_City-of-London-UK (2024-09-25)
CDSs for NosDFY and NosL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2171
Illegal BglII site found at 2959
Illegal BamHI site found at 1784 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 141
Illegal NgoMIV site found at 336
Illegal NgoMIV site found at 940
Illegal NgoMIV site found at 2034
Illegal NgoMIV site found at 2753
Illegal NgoMIV site found at 2971
Illegal NgoMIV site found at 3114
Illegal AgeI site found at 3194 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Coding sequences were codon-optimised for E. coli and illegal restriction sites were removed.
Please note, the CDS for NosD starts with the GTG codon.
Source
Sequences in the part are derived from P. Stutzeri Zobell