Part:BBa_K5111001
CDS for ComR (Ycfq)
When researching methods to increase the availability of copper in the cell, we came across a study which used a lux-based biosensor to monitor intracellular copper levels in situ, and a transposon mutagenesis approach to identify genes involved in copper entry into cells. They isolated mutant strains (called low-glowers) with reduced luminescence when exposed to copper, indicating lower intracellular copper levels. One of these mutants had a transposon insertion in the ComR gene, which encodes the ComR protein which is a TetR-like transcription regulator.
ComR did not regulate its own expression, but was required for copper-induction of the neighboring comC gene, as shown by real-time quantitative PCR and with a promoter-lux fusion. The purified ComR regulator bound to the promoter region of the comC gene in vitro and was released by copper. By membrane fractionation, ComC was shown to be present in the outer membrane. Cells lacking ComC showed increased copper accumulation in the periplasm and cytoplasm, evident due to the activation of CusRS (periplasmic) and CueR ( cytoplasmic).
Thus, ComC is an outer membrane protein which lowers the permeability of the outer membrane to copper. The expression of ComC is controlled by ComR, a novel, TetR-like copper-responsive repressor. While ComC expression is modulated by RpoE, CRP and ComR, it was found that ComR was the most effective in regulation. (https://pubmed.ncbi.nlm.nih.gov/22089859/)
The binding site for the ComR protein can be found here (https://parts.igem.org/Part:BBa_K5111000)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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