Protein_Domain

Part:BBa_K5109334:Design

Designed by: Veronika Rofner   Group: iGEM24_Uni-Padua-IT   (2024-09-28)


Anchoring motif OmpT truncated with linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NdeI cutting sites in order for its ATG to concide with the OmpT truncated's first codon.


Source

The sequence comes from E. coli. It has been extracted directly from the bacterial genome, performing a PCR with primers having a tail with NdeI and thrombin cutting sites.

References