Coding

Part:BBa_K5109015:Design

Designed by: Lisa Faccincani   Group: iGEM24_Uni-Padua-IT   (2024-09-26)


Multicopper Oxidase with His Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1504
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 209
    Illegal AgeI site found at 1086
    Illegal AgeI site found at 1351
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1


Design Notes

The enzymatic sequence has two different enzyme restriction sites at the ends: a BsaI restriction site upstream the coding sequence, and a BamHI restriction site downstream the sequence, after the His - Tag. Those sites have been designed in order to extract the Multicopper Oxidase sequence from the surface display system in which it is inserted (BBa_K5109022), in order to exchange it with other enzymes, without modifying the rest of the expression cassette. This part was designed for a multiple cloning experiment which involved exchanging it with three different enzymes, described in parts BBa_K5109013, BBa_K5109014, and BBa_K5109016.



Source

This part was identified with accurate bioinformatic research into Synechocystis sp genome. The sequence of the part was then reported on Benchling and synthezised.

References