DNA

Part:BBa_K5109002

Designed by: Lisa Faccincani   Group: iGEM24_Uni-Padua-IT   (2024-09-24)


DNA sequence for Golden Gate assembly.

This 32 bases sequence is a part that only contains two BsaI recognition sites, and was designed to be used to insert a terminator via golden gate assembly.

It is located downstream the coding sequence of an enzyme in an expression cassette, dehalogenase type II in part K5109001. It should be used for golden gate assembly only with elements that are designed with specific compatible ends leaved after BsaI cleaving. In this case, the sequence was designed to allow insertion of terminators from the 2024 distribution kit, that present compatible ends after cleavage with BsaI.

The part solves it's purpose when considered inside the complete context of use, in this case, part K5109901. In order to perform Golden Gate cloning on it, it should be located either into a plasmid, or into a sequence of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 27


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Parameters
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