Part:BBa_K5108006

aprA RBS from Pseudomonas protegens (CHA0)

Ribosome Binding Site of aprA gene from P. protogens

Contents
1. Usage and Biology
2. References

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

Ribosome Binding Site of aprA gene from P. protogens (CHAO). It was used for protein translation in P. fluorescens [1]. This RBS was synthesized in an operon with two genes and another RBS, and cloned into a plasmid. After transformation in P. fluorescens, our team had successful protein expression with this RBS. Check out composite part BBa_K5108009for more information.

References

1. Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073