RNA

Part:BBa_K5106014

Designed by: Ivar Gruppen   Group: iGEM24_WageningenUR   (2024-10-01)


TMSD threshold system output detector

Part of the components needed to establish a TMSD threshold system, designed by miRADAR (WageningenUR 2024), but can also act on its own as an RNA spinach aptamer. In the TMSD threshold system, this part is used to visualize the release of C. When an excess of A is present, it binds to all available B which releases C. When C is released, it can bind to D, and becomes fluorescent. For more information, look at https://2024.igem.wiki/wageningenur/part-collection, and the composite part, where this part is expressed under a T7 promoter. A more detailed explanation of the TMSD mechanism, as well as experimental results can be found on those pages

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 118
    Illegal NgoMIV site found at 135
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None