Composite

Part:BBa_K5102099:Design

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-10-01)


pU6_gRNA2-ProgRAM_tape5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

gRNA 2 encoded under the pU6 promoter with a 30 bp spacer, guiding PspCas13b-ADAR2DD complex to the second AUG in the ProgRAM_tape3, and the PspCas13b stem loop. During design of the part we ensured correct formation of gRNA hairpin and A to C mismatch in a target base which allows for higher deamination rate.


Source

Part assembled from the backbone available in lab and oligos from a DNA synthesis provider.

References