RNA

Part:BBa_K5102070:Design

Designed by: Fangting Zuo, Li Jiang, Ni Su, Yaqiang Zhang, Bingkun Bao, Limei Wang, Yajie Shi, Huimin Yang, Xinyi Huang, Ruilong Li, Qingmei Zeng, Zhengda Chen, Qiuning Lin, Yingping Zhuang, Yuzheng Zhao, Xianjun   Group: iGEM24_Munich   (2024-09-30)


4xdOkra


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 70
    Illegal XbaI site found at 159
    Illegal XbaI site found at 273
    Illegal XbaI site found at 362
    Illegal XbaI site found at 471
    Illegal XbaI site found at 556
    Illegal XbaI site found at 672
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 70
    Illegal XbaI site found at 159
    Illegal XbaI site found at 273
    Illegal XbaI site found at 362
    Illegal XbaI site found at 471
    Illegal XbaI site found at 556
    Illegal XbaI site found at 672
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 70
    Illegal XbaI site found at 159
    Illegal XbaI site found at 273
    Illegal XbaI site found at 362
    Illegal XbaI site found at 471
    Illegal XbaI site found at 556
    Illegal XbaI site found at 672
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the fluorescence of cells expressing mRNAs tagged with Okra substantially increased with higher copy numbers of aptamers in tandem, our design incorporated 4xdOkra.

Source

This part was ordered as a gBlock from a commercial DNA synthesis provider.

References

Zuo, F. et al. Imaging the dynamics of messenger RNA with a bright and stable green fluorescent RNA. Nat Chem Biol 1–10 (2024) doi:10.1038/s41589-024-01629-x.