RNA

Part:BBa_K5102018

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-09-30)


gRNA (30bp spacer) for switching state 0 to 1 for ProgRAM (tape 1)

The part encodes a guide RNA (gRNA) that functions to switch the state from 0 to 1 in the ProgRAM recorder. This gRNA directs a deactivated Cas13b protein from the Prevotella species, which is fused to the ADAR2 deaminase domain. The Cas13b-gRNA complex guides the deaminase to the target sequence, facilitating precise RNA editing for state change.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None