RNA
Part:BBa_K5102018
Designed by: Natalia Kuźmierkiewicz Group: iGEM24_Munich (2024-09-30)
gRNA (30bp spacer) for switching state 0 to 1 for ProgRAM (tape 1)
The part encodes a guide RNA (gRNA) that functions to switch the state from 0 to 1 in the ProgRAM recorder. This gRNA directs a deactivated Cas13b protein from the Prevotella species, which is fused to the ADAR2 deaminase domain. The Cas13b-gRNA complex guides the deaminase to the target sequence, facilitating precise RNA editing for state change.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |