Coding

Part:BBa_K5102014

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-09-30)


T2A

T2A peptide is a short sequence that causes ribosome skipping during translation, allowing the production of two distinct proteins from one mRNA strand. The mechanism is based on ribosomal pausing, which skips the formation of a peptide bond. It can be used to express multiple proteins from a single open reading frame. T2A protein sequence is derived from the Thosea asigna virus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None