Conjugation

Part:BBa_K5096400:Design

Designed by: Seonwoo Im   Group: iGEM24_Lambert-GA   (2024-10-01)


pCas9


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3478
    Illegal EcoRI site found at 7442
    Illegal XbaI site found at 1292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3478
    Illegal EcoRI site found at 7442
    Illegal NheI site found at 3237
    Illegal NheI site found at 6832
    Illegal NheI site found at 8024
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3478
    Illegal EcoRI site found at 7442
    Illegal BamHI site found at 5516
    Illegal BamHI site found at 6978
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3478
    Illegal EcoRI site found at 7442
    Illegal XbaI site found at 1292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3478
    Illegal EcoRI site found at 7442
    Illegal XbaI site found at 1292
    Illegal AgeI site found at 8110
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6571
    Illegal BsaI.rc site found at 6548


Design Notes

This part was originally developed by the Noireaux Lab.



Source

Streptococcus pyogenes

References