Coding

Part:BBa_K5094010:Design

Designed by: HuiChing Kuo   Group: iGEM24_KCIS-Xiugang-Taipei   (2024-09-24)


pGal1, 10-IsPETase (BBa_K5094001-BBa_K5094002)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 891
    Illegal AgeI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The team used BBa_K5094010 as a DNA template to make mutations but failed due to the large size of the pGal1,10 promoter in the ~7kb plasmid. The team will further optimize several primer sets of annealing conditions for the site-directed mutagenesis.


Source

The team used a traditional cloning technique to clone it downstream of the T7 promoter

References