Part:BBa_K5094005:Design

IsPETase/Thr116Ala/Lys259Glu, double mutations on the amino acids of 116 and 259 in the IsPETase gen

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 220
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The IsPETase gene has several repeat DNA regions so it might be difficult to mutate a specific amino acid via site-directed mutagenesis

Source

The team created the IsPETase/Thr116Ala/Lys259Glu, double mutations on the amino acids 116 and 259 in the IsPETase gene, via site-directed mutagenesis