Coding
Part:BBa_K5094003:Design
Designed by: HuiChing Kuo Group: iGEM24_KCIS-Xiugang-Taipei (2024-09-20)
IsPETase Thr116Ala, a single mutation on the amino acids of 116 in the IsPETase gene, amino acid 116
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 220
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The IsPETase gene has several repeat DNA regions so it might be difficult to create a mutation in your desire via site-directed mutagenesis
Source
the team used site-directed mutagenesis to create