Coding

Part:BBa_K5094003

Designed by: HuiChing Kuo   Group: iGEM24_KCIS-Xiugang-Taipei   (2024-09-20)


IsPETase Thr116Ala, a single mutation on the amino acids of 116 in the IsPETase gene, amino acid 116

IsPETaseThr116Ala, a single mutation on the amino acids of 116 in the IsPETase gene, amino acid 116 from the polar uncharged side chain of the Threonine switched to the hydrophobic side chain of the alanine, which would change the properties of the 116 amino acid, such as the interaction with the substrate or the catalytic function.

2024-09-25-124333.png

IsPETaseThr116Ala mutation involves a change at amino acid 116, where the polar uncharged threonine is replaced by the hydrophobic alanine. This alteration modifies the properties of amino acid 116, potentially affecting substrate interactions and altering the enzyme's catalytic function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None