Part:BBa_K5094001

An inducible Gal1, 10 promoter. The benefit of using the pGal1,10 promoter is to regulate the cloned gene’s expression highly efficiently by adding either glucose or galactose into the yeast medium to turn off or on the gene transcription ( 2022 ). Our team will use the pGal1, 10 promoter to do a yeast in vivo functional assay to determine if the IsPETase gene, along with variable mutations on the IsPETase gene cloned downstream of the pGal1,10 promoter, respectively, show different PET-degradation levels.