Composite

Part:BBa_K5091011:Design

Designed by: Angelina Oblak   Group: iGEM24_ULethbridge   (2024-10-01)


Native degQ Expression Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing the native degQ expression construct, several important factors should be considered. One of the key considerations was selecting an appropriate plasmid backbone. The plasmid’s copy number is important to balance the expression levels of degQ driven by its native promoter (BBa_K3429006) without causing stress to the host organism. A low or medium copy number plasmid would help maintain physiological expression levels similar to what is observed in Bacillus subtilis.

Another consideration was comparing the fengycin production levels between this native degQ construct and another degQ expression construct (BBa_K5091010), which uses a T7 promoter for stronger, induced expression. This comparison would help determine how much the native promoter impacts fengycin production versus the engineered T7-driven system.

These design considerations would provide insight into optimizing degQ expression for enhanced biocontrol applications while maintaining host cell viability.


Source

This is a composite part including the degQ protein coding region encoded in part BBa_5091005.