Composite

Part:BBa_K5091010:Design

Designed by: Angelina Oblak   Group: iGEM24_ULethbridge   (2024-10-01)


Deg Q Expression Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing the degQ expression construct, there are several important factors to consider. Selecting an appropriate plasmid is key, as the strength of the T7 promoter (BBa_I719005) combined with the plasmid’s copy number should balance gene expression without overburdening the host organism. A lower copy number plasmid may help reduce metabolic stress while still ensuring sufficient expression of the degQ gene.

Compatibility with T7 polymerase systems is crucial, as the T7 promoter requires the presence of T7 RNA polymerase for transcription. Additionally, using a strong ribosome binding site (BBa_B0034) ensures efficient translation, and a reliable terminator (BBa_B0010) ensures proper transcription termination and mRNA stabilization.


Source

This is a composite part including the degQ protein coding region encoded in part BBa_5091005.