Part:BBa_K5091010
Deg Q Expression Construct
This part is designed to express the degQ gene from Bacillus subtilis XF-1, which helps increase the production of fengycin, a lipopeptide known for its antifungal activity. The construct consists of the following components:
T7 Promoter (BBa_I719005): A strong promoter used for high-level expression in systems that have T7 RNA polymerase, like many engineered E. coli strains. This ensures the degQ gene gets transcribed efficiently.
RBS (BBa_B0034): A standard ribosome binding site that ensures the degQ gene is translated efficiently after being transcribed.
degQ Gene: The degQ gene encodes a regulatory protein that increases fengycin production. Fengycin is important for controlling fungal pathogens, which makes this part useful in agricultural projects focused on biocontrol.
T1 Terminator (BBa_B0010): A common terminator used to stop transcription, ensuring that the mRNA produced is properly processed and stable.
This part is ideal for projects that want to boost the production of fengycin in bacteria, particularly for applications in sustainable agriculture or biocontrol. By using the T7 promoter, this construct ensures strong expression of degQ in compatible systems, leading to increased production of fengycin to help protect plants from fungal and fungal-type pathogens.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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