Composite

Part:BBa_K5091007

Designed by: Zachary Robinson   Group: iGEM24_ULethbridge   (2024-09-22)


FAB with Addition of Chimeric Peptide

A FAB was created with the CDR3 regions containing a chimeric peptide that binds to our truncated PbEl04 protein (BBa_K4139011). Additionally, three tags were incorporated into each domain of the FAB: a histidine tag for easier purification, an OmpA tag for improved expression, and a poly-lysine tag to facilitate chemical binding with both our fluorescent probe and gold nanobodies.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2140
    Illegal PstI site found at 2700
    Illegal PstI site found at 2964
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2140
    Illegal PstI site found at 2700
    Illegal PstI site found at 2964
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2140
    Illegal PstI site found at 2700
    Illegal PstI site found at 2964
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2140
    Illegal PstI site found at 2700
    Illegal PstI site found at 2964
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 787
    Illegal AgeI site found at 2076
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None