Composite

Part:BBa_K5091006

Designed by: Zachary Robinson   Group: iGEM24_ULethbridge   (2024-09-22)


Nanobody Scaffold with Chimeric Peptide

A nanobody scaffold was created with the CDR3 region containing a chimeric peptide that binds to our truncated PbEl04 protein (BBa_K4139011). Additionally, three tags were incorporated into this construct: a histidine tag for easier purification, an OmpA tag for improved expression, and a poly-lysine tag to facilitate chemical binding with both our fluorescent probe and gold nanobodies.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 313
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 313
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 313
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 313
    Illegal NgoMIV site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None