Composite
Part:BBa_K5091006
Designed by: Zachary Robinson Group: iGEM24_ULethbridge (2024-09-22)
Nanobody Scaffold with Chimeric Peptide
A nanobody scaffold was created with the CDR3 region containing a chimeric peptide that binds to our truncated PbEl04 protein (BBa_K4139011). Additionally, three tags were incorporated into this construct: a histidine tag for easier purification, an OmpA tag for improved expression, and a poly-lysine tag to facilitate chemical binding with both our fluorescent probe and gold nanobodies.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 313
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 313
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 313
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 313
Illegal NgoMIV site found at 364 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |