Coding

Part:BBa_K5091005:Design

Designed by: Angelina Oblak   Group: iGEM24_ULethbridge   (2024-09-30)


degQ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For optimal use of the degQ gene from Bacillus subtilis XF-1, several design considerations should be taken into account. Using an inducible promoter allows for controlled expression, preventing potential metabolic burden or toxicity in the host organism. Employing a low copy number plasmid can further minimize stress on the host while maintaining stable gene expression. To optimize fengycin production, a system to accurately measure its levels, such as biochemical assays or HPLC, is recommended. Additionally, if expressing degQ in a different host organism, codon optimization should be considered to ensure efficient translation and protein production.


Source

Bacillus subtilis XF-1, sequence found through Genebank https://www.ncbi.nlm.nih.gov/nuccore/CP004019

References

Shengye Guo, Xingyu Li, Pengfei He, Honhing Ho, Yixin Wu, Yueqiu He, Whole-genome sequencing of Bacillus subtilis XF-1 reveals mechanisms for biological control and multiple beneficial properties in plants, Journal of Industrial Microbiology and Biotechnology, Volume 42, Issue 6, 1 June 2015, Pages 925–937, https://doi.org/10.1007/s10295-015-1612-y