Part:BBa_K5091005
degQ
The degQ gene from Bacillus subtilis XF-1 encodes a regulatory protein involved in enhancing the production of fengycin, a cyclic lipopeptide with potent antifungal activity. Fengycin synthesis is carried out by a cluster of non-ribosomal peptide synthetases (NRPSs), including the ppsA-ppsE gene cluster. The role of degQ is to upregulate the expression of sfp, a key gene required for fengycin biosynthesis, thereby increasing overall fengycin production.
Fengycin is particularly effective in suppressing the growth of filamentous fungi due to its structural properties, including cyclization, branching, and unusual amino acid constituents. It works by lysing fungal cells, and its antifungal activity has been demonstrated against the plant pathogen Plasmodiophora brassicae, a major cause of clubroot disease in crops. The production of fengycin by B. subtilis XF-1 makes this strain a valuable biological control agent in agricultural settings, offering an eco-friendly alternative to chemical pesticides.
In practical applications, introducing or overexpressing degQ in B. subtilis or other host organisms can be used to enhance fengycin production, thus improving the efficacy of biocontrol strategies against fungal pathogens. This makes degQ an attractive gene for projects focused on sustainable agriculture and plant protection.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |