Coding

Part:BBa_K5091000

Designed by: Ashkan Mosabeb-Omran   Group: iGEM24_ULethbridge   (2024-09-22)


Nanobody scaffold

This part is the nanobody scaffold developed by Mauricio Aguilar Rangel. This scaffold allows us to make a protein that binds to our target antigen with the addition of the binding sequence. Nanobodies are known as a single domain antibody that are significantly smaller and simpler than other antibody alternatives.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 242
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 242
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 242
    Illegal NgoMIV site found at 293
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None