Regulatory

Part:BBa_K5090010:Design

Designed by: Manuel Marques Vilar   Group: iGEM24_Stony-Brook   (2024-10-01)


BBa_J61100 + miRNA-326 Target Site (BBa_K5090006)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains the first 12 nucleotides of BBa_J61100 and 18 nucleotides of miRNA-326 (in between the middle and end of the 21-nucleotide sequence), in addition to the start codon. The rationale for which nucleotides were of each original part was based on maximizing the functionality of the mixed part while staying within the limits of bacterial gene expression. As such, we retained the original 12 nucleotides of BBa_J61100 to retain the ability of the ribosome to bind to the site as much as possible, while adding 18 nucleotides of miRNA-326 to ensure Argonaute 2, when expressed alongside a gene under this RBS, will still be able to bind to the sequence.



Source

New creation; portions of this part were sourced from BBa_J61100 and BBa_K5090006.

References