Composite

Part:BBa_K5089002:Design

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)


CLE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 226
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This expression system is codon optimized for expression in E.coli.


Source

CLE is a cutinase from Cryptococcus sp. strain S-2, previously shown to cleave carbon-chain ester substrates [1].

References

1.Masaki, K., Kamini, N. R, Ikeda, H., and Iefuji, H. 2005. Cutinase-Like Enzyme from the Yeast Cryptococcus sp. Strain S-2 Hydrolyzes Polylactic Acid and Other Biodegradable Plastics. Appl. and Environ. Microbiol., 71(11), 7548–7550.https://doi.org/10.1128/AEM.71.11.7548-7550.2005