Composite

Part:BBa_K5089001:Design

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)


ABO2449


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 812
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 462
    Illegal NgoMIV site found at 636
    Illegal NgoMIV site found at 977
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for this part is codon-optimized for expression in E. coli.


Source

Alcanivorax borkumensis, gene number 2449


References

1. Hajighasemi, M., et al., 2016. Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases. Biomacromolecules, 17(6), 2027–2039. https://doi.org/10.1021/acs.biomac.6b00223