Translational_Unit
virB1-11

Part:BBa_K5088705:Design

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-08-06)


TU virB1-11 from A. tumefaciens TiBo542


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2565
    Illegal EcoRI site found at 3963
    Illegal EcoRI site found at 5274
    Illegal EcoRI site found at 5686
    Illegal EcoRI site found at 8570
    Illegal PstI site found at 3463
    Illegal PstI site found at 4048
    Illegal PstI site found at 5099
    Illegal PstI site found at 7933
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2565
    Illegal EcoRI site found at 3963
    Illegal EcoRI site found at 5274
    Illegal EcoRI site found at 5686
    Illegal EcoRI site found at 8570
    Illegal NheI site found at 1920
    Illegal NheI site found at 8335
    Illegal NheI site found at 9478
    Illegal PstI site found at 3463
    Illegal PstI site found at 4048
    Illegal PstI site found at 5099
    Illegal PstI site found at 7933
    Illegal NotI site found at 4616
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2565
    Illegal EcoRI site found at 3963
    Illegal EcoRI site found at 5274
    Illegal EcoRI site found at 5686
    Illegal EcoRI site found at 8570
    Illegal BglII site found at 1666
    Illegal BglII site found at 2096
    Illegal BglII site found at 5815
    Illegal BglII site found at 6778
    Illegal BglII site found at 9575
    Illegal BamHI site found at 2334
    Illegal BamHI site found at 7480
    Illegal BamHI site found at 7680
    Illegal BamHI site found at 8616
    Illegal XhoI site found at 2301
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2565
    Illegal EcoRI site found at 3963
    Illegal EcoRI site found at 5274
    Illegal EcoRI site found at 5686
    Illegal EcoRI site found at 8570
    Illegal PstI site found at 3463
    Illegal PstI site found at 4048
    Illegal PstI site found at 5099
    Illegal PstI site found at 7933
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2565
    Illegal EcoRI site found at 3963
    Illegal EcoRI site found at 5274
    Illegal EcoRI site found at 5686
    Illegal EcoRI site found at 8570
    Illegal PstI site found at 3463
    Illegal PstI site found at 4048
    Illegal PstI site found at 5099
    Illegal PstI site found at 7933
    Illegal NgoMIV site found at 1229
    Illegal NgoMIV site found at 1405
    Illegal NgoMIV site found at 4592
    Illegal NgoMIV site found at 5560
    Illegal NgoMIV site found at 5570
    Illegal NgoMIV site found at 7505
    Illegal NgoMIV site found at 8347
    Illegal AgeI site found at 663
    Illegal AgeI site found at 2228
    Illegal AgeI site found at 4379
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2174


Design Notes

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Source

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References