Part:BBa_K5087028

T7 promotor template

This part is a template for the T7 promoter, meaning: a sequence complementary to the T7 promoter that serves as a foundational building block for IVT (in vitro transcription) templates used to synthesise crRNA molecules, which are crucial for use in the SHERLOCK [1] system (Specific High-sensitivity Enzymatic Reporter unLOCKing).

Not only is this sequence a key building block for such templates, but it also forms the core of the SynLOCK system developed by the 2024 JU-Krakow iGEM team. The SynLOCK Cassette (BBa_K5087017), which enables the creation of custom crRNA molecules compatible with the LwaCas13a protein, includes this part.

Sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

• [1] Kellner, Max J., Jeremy G. Koob, Jonathan S. Gootenberg, Omar O. Abudayyeh, and Feng Zhang. “SHERLOCK: Nucleic Acid Detection with CRISPR Nucleases.” Nature Protocols 14, no. 10 (October 2019): 2986–3012. https://doi.org/10.1038/s41596-019-0210-2